Specific and Nonspecific Hybridization of Oligonucleotide Probes on Microarrays
نویسندگان
چکیده
منابع مشابه
Specific and nonspecific hybridization of oligonucleotide probes on microarrays.
Gene expression analysis by means of microarrays is based on the sequence-specific binding of RNA to DNA oligonucleotide probes and its measurement using fluorescent labels. The binding of RNA fragments involving sequences other than the intended target is problematic because it adds a chemical background to the signal, which is not related to the expression degree of the target gene. The artic...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2005
ISSN: 0006-3495
DOI: 10.1529/biophysj.104.055343